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PROBER(TM) Probe DNA Purifying System

CAT. 17072 (100 rxn.)

[We are pleased to offer unpublished higher discounts on large volume purchases.]

PROBER(TM) System is designed for removal of unincorporated labeled nucleotides following
end-labeling, probe labeling, or unincorpo-rated dye terminators from sequencing.


For the experimental procedure such as Northern Blot or in situ hybridization, DNA labeling with isotope of chemiluminescent dye is essential. Usually, unincorporated labeled nucleotides, such as dNTP, random primer, isotope or dye, are following end-labeling, probe labeling, or polymerization reaction with labeled nucleotides. These residues may cause non-specific binding or background signal; therefore, they should be removed in order to achieve satisfactory results. The iNtRON PROBER(TM) Probe DNA Purifying System is pre-packed column useful for the purification of probe DNA for Southern hybridization, Northern hybridization, and in situ hybridization.

Fast procedure with only two short centrifugation steps
Ready-to-use Sephadex resin
Column is filled with sephadex resin

Format : Sephadex resin type
Application: Probe DNA purification, dye removal, or removal of unincorporated labeled nucleotides
Recovery: >95%
Elution range: >20bp DNA or 20mer oligonucleotide
Prep. time: <5 minutes


Included in this kit
; PROBER(TM) columns containing Sephadex resin, and a manual.

 

 

 

 

 

 

 
 
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