Protein Related Products >
Protein Measurement Kit
CAT. 21011 (100 ml)
21012 (200 ml)
[We are pleased to offer unpublished
higher discounts on large volume purchases.]
quantification is one of the important steps in protein research
such as Western blot assay. Protein assay methods are diverse,
and there is one which absorbs at 280nm without special reagents
or operation. This method is simple, yet it also has a shortcoming
not being able to analyze protein assay when no amino acid
such as phenylalanine, tryptophan or tyrosine are present.
Neither will it be used commonly due to its DNA absorbance
interference (AI). The other methods are Lowry assay or BCA
assay, which are highly sensitive for protein quantification,
but is quite inconvenient and time-consuming. Contrarily,
Bradford method is the most commonly used one (within 10min),
which can detect the minimum amount of protein and is very
simple to use. This PRO-MEASURE(TM) is even more convenient
than Bradford assay, declining AI of solution itself so that
the background absorbance is
maintained very low.
Suitable for PRO-PREP(TM) Kit and WEST-ZOL(TM)
High sensitivity and specificity (10-25ng/ml)
time (<10 min)
Standard solution (BSA solution) supplied.
: PRO-MEASURE(TM) solution,
BSA standard (1mg/ml)
Before use, dilute 1 part of PRO-MEASURE(TM)
solution by adding 9 parts of sterile water (working solution).
Store at 4,
and then stable for over 1 year.
Included in this kit
; reagents, and a manual.