DNA/RNA Purification Related Products >
MEGA-bead(TM) Agarose Gel DNA
17011 (100 rxn.)
[We are pleased
to offer unpublished higher discounts on
large volume purchases.]
DNA fragments for probe
DNA or ligation must be separated and purified
from other DNA fragments. MEGA-bead(TM)
employs a bead method to purify target DNA
in excised agarose gel. The bead method
uses a highly concentrated salt solution
to keep the target DNA bound to the bead
and prevent the target DNA from solubilization.
The binding reaction occurs due to the disruption
of the organized structure of water molecules
and the interaction with the nucleic acids.
Thus the adsorption to the specifically
pretreated spherical silica matrix is favored.
Since the binding process is specific for
nucleic acids, the bound material can be
separated and purified from impurities e.g.
salts and proteins, by a simple washing
step. Nucleic acids elute from the matrix
in a low salt buffer or water.
size of MEGA-bead(TM) is much smaller than
other companies' products to yield more
surface area for DNA binding, and guarantees
high yield of purification up to 80-90%.
DNA fragments isolated with MEGA-bead(TM)
Agarose Gel Extraction Kit are efficiently
ligated into plasmid cloning vectors or
specifically labeled using either random
primed labeling or nick translation. No
inhibition of digestion with restriction
endonucleases is observed. Within
minutes of procedure, pure DNA can be obtained.
DNA fragments extracted in
extraction from standard or low-melt gels
in TAE or TBE buffer
use sodium iodide.
of pH, then needn¡¯t add sodium acetate.
High recovery and purity
No interfere with subsequent
Format : Bead
extraction or enzymatic reaction cleanup
in 500bp - 10Kb
DNA Binding capacity: 4-5mg
Prep. time: <20
Rehydration volume: 20-50ml
Included in this kit
Beads, reagents, buffers, and a manual.