PCR Related Products >
Mycoplasma Detection PCR Premix Kit
25091 (48 rxn.)
[We are pleased
to offer unpublished higher discounts on
large volume purchases.]
Mycoplasma are common and
serious contaminants of cell cultures. It
has been shown that 30-87% of cell cultures
are infected with mycoplasma. Many Mycoplasma
contaminations, particulary in continuous
cell lines, grow slowly and do not destroy
host cells but are still able to affect
various parameters, leading to unreliable
or false results. These effects include
changes in metabolism, growth, viability,
DNA, RNA and protein synthesis, morphology
etc.. Testing for mycoplasma is essential
quality control tool to assure accurate
research and reliable biotechnological products.
The Primers used in the kits anneal to conserved
regions of the mycoplasma genome, allowing
the detection of all several species of
mycoplasma. Because mycoplasma contaminants
can be detected by simply verifying amplification
DNA fragments on an agarose gel, there is
no need to prepare probes labeled with radioisotope.
Each tube of i-Myco PCR Mycoplasma
Detection Kit contains all components for
PCR, i-Taq(TM) DNA polymerase, dNTPs buffer
and primers for Mycoplasma partial gene
amplifications except template DNA.
Sensitivity: The kit allows the detection
of at least 10-infected cell suspension
or 10ng genomic DNA.
all mycoplasma using one and the same protocol,
as opposed to the culture method that requires
different culture conditions for different
whole procedure can be performed within
a few hour by One-tube reaction mix
Broad Detection Range: The
Primers, used in the kit hybridize to conserved
regions of the genome and allow the detection
of all Mycoplasma species.
Format : Lyophilized
PCR premix containing i-Taq(TM) DNA polymerase,
PCR buffer, enhancing buffer, loading dye,
and primers set for detecting Mycoplasma.
adding a template into a tube, then add
water up to 20ml.
at -20, and then stable
for over 1 year.
in this kit
; Tubes containing lyophilized
premix reagents, and a manual.
PCR process is covered by patents issued
and applicable in certain countries. iNtRON
Biotechnology does not encourage or support
the unauthorized or unlicensed use of the
PCR process. Use of this product is recommended
for persons that either have a license to
perform PCR or are not required to obtain