PCR Related Products >
25021 (250 Units)
25022 (500 Units)
[We are pleased
to offer unpublished higher discounts on
large volume purchases.]
PCR (polymerase chain reaction)
is developed by Kary Mullis in mid 1980's
and it has made development of modern molecular
biology possible through DNA oligo sequence.
The commonly used DNA polymerase in PCR
method is Taq DNA polymerase. In the beginning,
the enzyme used in PCR method was E.
coli DNA polymerase, but the
enzyme had to be added at every step of
the process due to its thermal instability.
Therefore, DNA polymerase is developed from
Thermus aquaticus bacteria which thrives
in hot spa. Taq DNA polymerase optimally
compose DNA at 72, therefore
it could stably amplify a specified oligo
sequence without adding enzyme at every
cycle due to its thermal stability even
polymerase is the recombinant form of the
enzyme from the thermophilic Thermus aquaticus,
expressed in E. coli. The enzyme is highly
purified and is free of non-specific endonuclease
or exonuclease. Taq DNA polymerase consists
of a single polypeptide chain with a molecular
weight of approximately 95kD. It is a highly
processive 5'-3' DNA polymerase lacking
3'-5' exonuclease activity.
efficiency of the amplification
Not contaminated with E.coli
Format : i-Taq(TM)
DNA polymerase, PCR buffer (w/ 15mM MgCl2),
MgCl2 free PCR buffer, 10mM dNTPs, and 25mM
i-Taq(TM) DNA polymerase
definition: One unit is defined as
the amount of enzyme required to catalyze
the incorporation of 10nmol of dNTP into
an acid-insoluble form in 30 minutes at
74. The reaction conditions
are in upper PCR working buffer, and 200mM
dNTPs, and radiolabeled dTTP, and 150mg/ml
activated calf thymus DNA.
at -20, and then stable
for over 1 year.
in this kit
; reagents, and a manual.
PCR process is covered by patents issued
and applicable in certain countries. iNtRON
Biotechnology does not encourage or support
the unauthorized or unlicensed use of the
PCR process. Use of this product is recommended
for persons that either have a license to
perform PCR or are not required to obtain