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i-Taq(TM) DNA Polymerase

CAT. 25021 (250 Units)
CAT. 25022 (500 Units)
[We are pleased to offer unpublished higher discounts on large volume purchases.]

PCR (polymerase chain reaction) is developed by Kary Mullis in mid 1980's and it has made development of modern molecular biology possible through DNA oligo sequence. The commonly used DNA polymerase in PCR method is Taq DNA polymerase. In the beginning, the enzyme used in PCR method was E. coli DNA polymerase, but the enzyme had to be added at every step of the process due to its thermal instability.
Therefore, DNA polymerase is developed from Thermus aquaticus bacteria which thrives in hot spa. Taq DNA polymerase optimally compose DNA at 72, therefore it could stably amplify a specified oligo sequence without adding enzyme at every cycle due to its thermal stability even at 94.

Taq DNA polymerase is the recombinant form of the enzyme from the thermophilic Thermus aquaticus, expressed in E. coli. The enzyme is highly purified and is free of non-specific endonuclease or exonuclease. Taq DNA polymerase consists of a single polypeptide chain with a molecular weight of approximately 95kD. It is a highly processive 5'-3' DNA polymerase lacking 3'-5' exonuclease activity.

High efficiency of the amplification
Not contaminated with E.coli genomic DNA
High purity
Supplied dNTPs

Format : i-Taq(TM) DNA polymerase, PCR buffer (w/ 15mM MgCl2), MgCl2 free PCR buffer, 10mM dNTPs, and 25mM MgCl2
Unit: 5U/ml i-Taq(TM) DNA polymerase
Unit definition: One unit is defined as the amount of enzyme required to catalyze the incorporation of 10nmol of dNTP into an acid-insoluble form in 30 minutes at 74. The reaction conditions are in upper PCR working buffer, and 200mM dNTPs, and radiolabeled dTTP, and 150mg/ml activated calf thymus DNA.
Stability: Store at -20, and then stable for over 1 year.

Included in this kit
; reagents, and a manual.

The PCR process is covered by patents issued and applicable in certain countries. iNtRON Biotechnology does not encourage or support the unauthorized or unlicensed use of the PCR process. Use of this product is recommended for persons that either have a license to perform PCR or are not required to obtain a license.