PCR Related Products >
25161 (250 Units)
25162 (500 Units)
[We are pleased
to offer unpublished higher discounts on
large volume purchases.]
i-StarTaq(TM) DNA polymerase,
a modified system of i-Taq(TM) DNA polymerase,
is supplied in an inactive state that has
no polymerase activity at ambient temperature.
This system is a premixed complex of i-Taq(TM)
DNA polymerase and the enhanced
that inhibit the DNA polymerase activities
at ambient temperature.
extension of nonspecifically annealed primers
and primer-dimer formed
at low temperatures
during PCR reaction.
DNA polymerase shows a stringent PCR specificity
and reproducibility assay in which low-copy
template targets are amplified efficiently.
PCR (polymerase chain reaction) is
developed by Kary Mullis in mid 1980's and
it has made development of modern molecular
biology possible through DNA oligo sequence.
The commonly used DNA polymerase in PCR
method is Taq DNA polymerase. In the beginning,
the enzyme used in PCR method was E.
coli DNA polymerase, but the
enzyme had to be added at every step of
the process due to its thermal instability.
Therefore, DNA polymerase is developed from
Thermus aquaticus bacteria which thrives
in hot spa. Taq DNA polymerase optimally
compose DNA at 72, therefore
it could stably amplify a specified oligo
sequence without adding enzyme at every
cycle due to its thermal stability even
fidelity, fast extension speed, and outstanding
accuracy and yield
amplifies GC-rich sequences
mispriming and primer-dimer
mix, 10x PCR buffer (Mg2+), 10x PCR buffer,
Format : i-StarTaq(TM)
DNA polymerase, PCR buffer (w/ 15mM MgCl2),
MgCl2 free PCR buffer, 10mM dNTPs, and 25mM
i-MAX(TM) DNA polymerase
definition: One unit is defined as
the amount of enzyme required to catalyze
the incorporation of 10nmol of dNTP into
an acid-insoluble form in 30 minutes at
74. The reaction conditions
are in upper PCR working buffer, and 200mM
dNTPs, and radiolabeled dTTP, and 150mg/ml
activated calf thymus DNA.
at -20, and then stable
for over 1 year.
in this kit
; reagents, and a manual.
PCR process is covered by patents issued
and applicable in certain countries. iNtRON
Biotechnology does not encourage or support
the unauthorized or unlicensed use of the
PCR process. Use of this product is recommended
for persons that either have a license to
perform PCR or are not required to obtain