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i-MAX(TM) DNA Polymerase

CAT. 25041 (250 Units)

[We are pleased to offer unpublished higher discounts on large volume purchases.]

i-MAX(TM) PCR System is a versatile and easy-to-use enzyme, with powerful advantages for all PCR applications. i-MAX(TM) PCR System is an optimized mixture of i-Taq(TM) DNA polymerase and a proofreading DNA polymerase which have been shown to significantly increase PCR product yield and enhance amplification of a broader range of targets compared to single enzyme formulations.
The enhanced performance of polymerase mixtures is presumably due to the capacity of one enzyme to complement the inability of a second enzyme to extend a primer through certain obstructions. These potential obstructions include mispaired bases that cause non-proofreading polymerases to stall prematurely and disassociate from the primer-template, basic gaps that cannot be bridged by polymerases lacking terminal transferase activity and template secondary structures such as GC-rich hairpins.

PCR (polymerase chain reaction) is developed by Kary Mullis in mid 1980's and it has made development of modern molecular biology possible through DNA oligo sequence. The commonly used DNA polymerase in PCR method is Taq DNA polymerase. In the beginning, the enzyme used in PCR method was
E. coli DNA polymerase, but the enzyme had to be added at every step of the process due to its thermal instability.
Therefore, DNA polymerase is developed from Thermus aquaticus bacteria which thrives in hot spa. Taq DNA polymerase optimally compose DNA at 72, therefore it could stably amplify a specified oligo sequence without adding enzyme at every cycle due to its thermal stability even at 94.

Ideal for amplification of large DNA fragments
Proofreading function
Amplifies DNA templates over 15Kb
Successfully amplifies GC-rich sequences
dNTP mix, 10x PCR buffer (Mg2+), 10x PCR buffer, Mg2+ supplied

Format : i-MAX(TM) DNA polymerase, PCR buffer (w/ 15mM MgCl2), MgCl2 free PCR buffer, 10mM dNTPs, and 25mM MgCl2
Unit: 5U/ml i-MAX(TM) DNA polymerase
Unit definition: One unit is defined as the amount of enzyme required to catalyze the incorporation of 10nmol of dNTP into an acid-insoluble form in 30 minutes at 74. The reaction conditions are in upper PCR working buffer, and 200mM dNTPs, and radiolabeled dTTP, and 150mg/ml activated calf thymus DNA.
Stability: Store at -20, and then stable for over 1 year.





Included in this kit
; reagents, and a manual.

The PCR process is covered by patents issued and applicable in certain countries. iNtRON Biotechnology does not encourage or support the unauthorized or unlicensed use of the PCR process. Use of this product is recommended for persons that either have a license to perform PCR or are not required to obtain a license.

 

 

 

 

 

 

 

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