PCR Related Products >
25041 (250 Units)
[We are pleased
to offer unpublished higher discounts on
large volume purchases.]
i-MAX(TM) PCR System is
a versatile and easy-to-use enzyme, with
powerful advantages for all PCR applications.
i-MAX(TM) PCR System is an optimized mixture
of i-Taq(TM) DNA polymerase and a proofreading
DNA polymerase which have been shown to
significantly increase PCR product yield
and enhance amplification of a broader range
of targets compared to single enzyme formulations.
The enhanced performance of polymerase mixtures
is presumably due to the capacity of one
enzyme to complement the inability of a
second enzyme to extend a primer through
certain obstructions. These potential obstructions
include mispaired bases that cause non-proofreading
polymerases to stall prematurely and disassociate
from the primer-template, basic gaps that
cannot be bridged by polymerases lacking
terminal transferase activity and template
secondary structures such as GC-rich hairpins.
PCR (polymerase chain reaction) is
developed by Kary Mullis in mid 1980's and
it has made development of modern molecular
biology possible through DNA oligo sequence.
The commonly used DNA polymerase in PCR
method is Taq DNA polymerase. In the beginning,
the enzyme used in PCR method was E.
coli DNA polymerase, but the
enzyme had to be added at every step of
the process due to its thermal instability.
Therefore, DNA polymerase is developed from
Thermus aquaticus bacteria which thrives
in hot spa. Taq DNA polymerase optimally
compose DNA at 72, therefore
it could stably amplify a specified oligo
sequence without adding enzyme at every
cycle due to its thermal stability even
for amplification of large DNA fragments
Amplifies DNA templates
amplifies GC-rich sequences
mix, 10x PCR buffer (Mg2+), 10x PCR buffer,
Format : i-MAX(TM)
DNA polymerase, PCR buffer (w/ 15mM MgCl2),
MgCl2 free PCR buffer, 10mM dNTPs, and 25mM
i-MAX(TM) DNA polymerase
definition: One unit is defined as
the amount of enzyme required to catalyze
the incorporation of 10nmol of dNTP into
an acid-insoluble form in 30 minutes at
74. The reaction conditions
are in upper PCR working buffer, and 200mM
dNTPs, and radiolabeled dTTP, and 150mg/ml
activated calf thymus DNA.
at -20, and then stable
for over 1 year.
in this kit
; reagents, and a manual.
PCR process is covered by patents issued
and applicable in certain countries. iNtRON
Biotechnology does not encourage or support
the unauthorized or unlicensed use of the
PCR process. Use of this product is recommended
for persons that either have a license to
perform PCR or are not required to obtain