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b-Gal Staining Kit

CAT. 21031 (150 rxn.)
[We are pleased to offer unpublished higher discounts on large volume purchases.]

b-Galactosidase is an enzyme that catalizes the hydrolysis of b-galactoside. The b-galactosidase gene (LacZ) is a bacterial gene often used as a reporter construct in eukaryotic transfection experiments for two reason : its protein product is extremly stable and resistant to proteolytic degradation, and most importantly, the enzyme activity is assayed easily. The b-Gal Staining Kit from iNtRON provides an easy and rapid method to determine transfection efficiency by indicating b-galactosidase activity in individual intact cells.
After transfecting cells with a plsamid expressing LacZ, the cells are incubated with a glutaraldehyde-formaldehyde fixing solution and then with a staining solution that contains X-Gal. In trasfected cells,
b-galactosidase cleaves X-Gal to produce a blue stain. The transfection efficiency is determined by counting stained and unstained cells under a microscope and calculating the percentage of stained cells in the total population.

Supplied all reagents for assaying b-galactosidase activity
High sensitivity and specificity

Very simple and convenient steps
Available of variable types of cells

Format : PBS (10x), Staining solution (10x), Fixative solution (10x), X-Gal (40mg/ml)
Sample size: Sufficient for 150 standard assay in 35mm (or 6-well) culuture dishes
Stability: Store at -20 without X-Gal solution, and then stable for over 6 months.

Included in this kit
; reagents, and a manual.







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