Protein Related Products >
CAT. 21031 (150 rxn.)
[We are pleased to offer unpublished
higher discounts on large volume purchases.]
is an enzyme that catalizes the hydrolysis of b-galactoside.
gene (LacZ) is a bacterial gene often used as a reporter construct
in eukaryotic transfection experiments for two reason : its
protein product is extremly stable and resistant to proteolytic
degradation, and most importantly, the enzyme activity is
assayed easily. The b-Gal
Staining Kit from iNtRON provides an easy and rapid method
to determine transfection efficiency by indicating b-galactosidase
activity in individual intact cells.
After transfecting cells with a plsamid expressing LacZ, the
cells are incubated with a glutaraldehyde-formaldehyde fixing
solution and then with a staining solution that contains X-Gal.
In trasfected cells, b-galactosidase
cleaves X-Gal to produce a blue stain. The transfection efficiency
is determined by counting stained and unstained cells under
a microscope and calculating the percentage of stained cells
in the total population.
Supplied all reagents for assaying b-galactosidase
sensitivity and specificity
Very simple and convenient steps
Available of variable types of cells
: PBS (10x), Staining solution
(10x), Fixative solution (10x), X-Gal (40mg/ml)
Sufficient for 150 standard assay in
35mm (or 6-well) culuture dishes
Store at -20
without X-Gal solution, and then stable for over 6 months.
Included in this kit
; reagents, and a manual.