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Nitric Oxide (NO) Detection Kit

CAT. 21021 (<1,000 assays)
CAT. 21022 (<2,000 assays)
[We are pleased to offer unpublished higher discounts on large volume purchases.]

Nitric oixde (NO-) is radical produced during the transformation of L-arginine to citrulline by nitric oxide synthase (NOS).
The Nitric Oxide Detection Kit from iNtRON, based on diazotization (Griess method) assay can detect in vitro NO concentration. The kit enables the researchers to overcome difficulties in detecting NO concentration due to the short half life (about 5 seconds) of gaseous
nitric oxide.
The kit will accurately detect the concentration of NO by indirectly measuring nitrite (NO2-), which is by-product of nitric oxide trans- formation in living tissue. The kit is based on the colorimetric change, which occurs when naphthylethylenediamine (NED) is added to the by-product of reaction between sulfanilamide and nitrite. The limit of detection is 2.0mM nitrite in ultrapure distilled water using the recommended protocol.

N1 buffer (Substrate solution)
: Sulfanilamide in the reaction buffer
N2 buffer (Coloring solution)
: NED in the stabilizer buffer
Nitrite standard, 1mM
Store at 4 .

Included in this kit
; reagents, and a manual.







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