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Oxide (NO) Detection Kit
CAT. 21021 (<1,000 assays)
21022 (<2,000 assays)
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oixde (NO-) is radical produced during the transformation
of L-arginine to citrulline by nitric oxide synthase (NOS).
The Nitric Oxide Detection Kit from iNtRON, based on diazotization
(Griess method) assay can detect in vitro NO concentration.
The kit enables the researchers to overcome difficulties in
detecting NO concentration due to the short half life (about
5 seconds) of gaseous
The kit will accurately detect the concentration of NO by
indirectly measuring nitrite (NO2-), which is by-product of
nitric oxide trans- formation in living tissue. The kit is
based on the colorimetric change, which occurs when naphthylethylenediamine
(NED) is added to the by-product of reaction between sulfanilamide
and nitrite. The limit of detection is 2.0mM nitrite in ultrapure
distilled water using the recommended protocol.
N1 buffer (Substrate solution)
: Sulfanilamide in the reaction buffer
: NED in the stabilizer buffer
at 4 .
Included in this kit
; reagents, and a manual.